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Specific binding proteins to TG09-02-immobilized magnetic nanobeads using SDS-PAGE. TG09-02 was covalently conjugated to FG beads and incubated with protein fractions of HT29 cells. The bound proteins were eluted and subjected to SDS gel electrophoresis and silver staining. The specific binding proteins were divided into the six areas #1–#6 and then applied to LC-MS/MS analysis. M protein marker
