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. Author manuscript; available in PMC: 2015 Jan 8.

Published in final edited form as: J Nutr Biochem. 2007 Mar 23;18(9):597–608. doi: 10.1016/j.jnutbio.2006.11.005

Fig. 1 — Chromatography of ⁶⁷Cu-labeled rat plasma extracts during purification of transcuprein by Sephadex G150 (A), Sephacryl S300 (B), and copper chelate affinity chromatography (C), showing absorbance at 280 nm (solid line), and ⁶⁷Cu radioactivity (black diamonds) or actual Cu concentrations determined by atomic absorption spectrometry (X---X, or black dots in C). (C) Elution of proteins bound to the copper chelate affinity gel, upon application of 20 ml of a 10–40 mM imidazole gradient (up-angled line), followed by an additional 20 ml of 40 mM imidazole.

Fig. 1 — Chromatography of ⁶⁷Cu-labeled rat plasma extracts during purification of transcuprein by Sephadex G150 (A), Sephacryl S300 (B), and copper chelate affinity chromatography (C), showing absorbance at 280 nm (solid line), and ⁶⁷Cu radioactivity (black diamonds) or actual Cu concentrations determined by atomic absorption spectrometry (X---X, or black dots in C). (C) Elution of proteins bound to the copper chelate affinity gel, upon application of 20 ml of a 10–40 mM imidazole gradient (up-angled line), followed by an additional 20 ml of 40 mM imidazole.