Fig. 4.

Elution of transcuprein during purification by published methods used for α₁inhibitorIII [29]. The flow-through from Cibacron blue chromatography of radiolabeled rat serum was applied to ion exchange chromatography on DEAE Sepharose and washed with 50 mM NaCl-Tris buffer. What is shown is the elution of proteins (absorbance at 280 nm; solid line) and copper radioactivity (black diamonds) from the ion exchange column, upon application of the NaCl gradient (100–400 mM). Pooled eluate (from 15–24 ml) was then applied to a Sephacryl S300 column (not shown). SDS-PAGE analysis of the resulting preparation is in Fig. 2A, 4^th example.