Fig. 5.

Copper binding to human α₂macroglobulin. To demonstrate that α₂macroglobulin is the human transcuprein, traces of ⁶⁴Cu(II) were added to samples of pooled human plasma. Samples on the left half of the gel were from normal subjects; those on the right from two patients with iron overload (hemochromatosis). Portions (6 μL) of undiluted (larger rockets) or ten-fold diluted plasma (smaller rockets) were applied to adjacent wells in triplicate. The resulting gels were developed by autoradiography in a phosphorimager, detecting the ⁶⁴Cu.