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. Author manuscript; available in PMC: 2015 Jan 8.
Published in final edited form as: J Nutr Biochem. 2007 Mar 23;18(9):597–608. doi: 10.1016/j.jnutbio.2006.11.005

Fig. 8.

Fig. 8

Fig. 8

Fig. 8

Fig. 8

Effects of copper deprivation and iron status on expression of α1inhibitorIII (α1I3) mRNA (transcuprein) (Tc/α1I3), determined by Northern analysis. (A) Representative nylon membrane of total RNA, after capillary transfer from agarose gel containing ethidium bromide, showing the loading of RNA from copper-normal and copper deficient rat livers. (B) Portions of the resulting Northern autoradiograph, showing hybridization with Tc/α1I3 cDNA and that of the glyceraldehyde-3-phosphate dehydrogenase (G3PDH) control, for copper-normal and deficient rats, as indicated. (C) Northern of liver and heart total RNA from rats with normal (Norm.), deficient (Defic.) and excess (Xcess) iron status, showing expression of Tc/α1I3 mRNA above 28 S rRNA migration mark. (D) Northern of total liver RNA from normal and iron deficient rats, showing hybridization with Tc/α1I3 and G3PDH, respectively.