Expression of transcuprein protein in serum of rats with varying iron and copper status, determined by Western blotting. (A) Expression of the α1inhibitorIII (190 kDa) component in iron deficient (lanes 1,2), normal (lanes 3,4), and excess iron (lanes 5,6) treated rats. Equal (5 μL) portions of plasma were analyzed. (B) Expression in copper deficient (D) and supplemented (N) rats.