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Binding of Grb-2 and GADS to CD28. Jurkat T-cells, either left unstimulated (lane 2) or stimulated with anti-CD28/rabbit anti-mouse IgG for 1, 5, 15 and 30 min (lanes 3-6) were lysed, immunoprecipitated with anti-CD28 and blotted for Grb-2 (a) or GADS (b, c). Immunoprecipitation with rabbit anti-mouse IgG served as a negative control (lane 1). Input panels: blotting of cell lysates with Grb-2 or Gads served as a loading control.
