Figure 2.

(a) Plots of maximum height (normalized at 3 MPa) of the crosspeaks in ¹H/¹³C HSQC for L99A, obtained at various pressures, from 3 to 300 MPa. Changes in the protein concentration as a function of pressure are corrected, based on the pressure-induced compaction of the solvent water (by ≈9% at 300 MPa) (63). The crosspeaks of the side-chain methyl groups can be classified mainly into three groups: 1) the rapidly decaying group (red lines); 2) the intermediate decaying group (yellow lines); and 3) the slow decaying group (black lines). The rapid and the intermediate decaying groups comprise 1), I78δ₁, I78γ₂, L84δ₁, A99β, M102ε, L118δ₂, L121δ₁, and L133δ₂ and 2), I3δ₁, I3γ₂, M6ε, L7δ₁, I29δ₁, I29γ₂, L46δ₁, L66δ₁, V71γ₂, V75γ₂, L84δ₂, V87γ₂, A98β, I100γ₂, M106ε, A129β, A130β, I150δ₁, and I150γ₂, respectively. Errors in height estimation are presented in Fig. S3. (b) The observable side-chain methyl groups in the HSQC spectrum at 3 MPa (Fig. 1A) are depicted with spheres. Those belonging to the rapid and intermediate decaying groups are represented by red and yellow spheres, respectively, on the tertiary structure, and residues located around the internal cavities are designated by their residue number. The picture was prepared using MOLMOL (54). To see this figure in color, go online.