Figure 3.

Distribution of residues based on (a) ¹³C chemical shift differences (|Δω|) for side-chain methyl groups between exchanging sites, as estimated by the R₂ dispersion NMR experiments for L99A (31). The amplitude of |Δω| is represented by a color scale from yellow to red. (b) Side-chain methyl groups showing significant loss of their crosspeak intensities at a high pressure. The pressure at which the relative peak intensity is reduced to <0.6 from the original is represented by colors: <200 MPa; red, 250 MPa; orange, and 300 MPa; yellow. (c) Distribution of residues based on the ¹H chemical shift differences (|Δω|) for side-chain methyl groups between exchanging sites, as estimated by the ZZ-exchange NMR experiments for L99A/G113A (32). The picture was prepared using MOLMOL (54). To see this figure in color, go online.