Figure 3.

Two-dimensional SAMPI4 spectra of Pf1 coat protein reconstituted in DMPC (top) and DOPC/DOPG at 9:1 molar ratio (bottom) and aligned by AAO are overlaid with least-squares PISA wheels. Positions of the centers of NMR intensities for the individual peaks corresponding to nonglycine residues (shown as red crosses) were least-squares fitted to a PISA wheel (continuous red lines); fits to glycines (shown as blue crosses) are also given (dashed blue lines). Spectra were recorded at T = 45°C and B₁= 51 kHz with 1024 scans used for each of the 64 t₁ increments. To see this figure in color, go online.