Fig. 2.

Double immunolabeling for T-type calcium channels in cell cultures from non-pregnant/pregnant myometrium. a Ca_V3.1 immunolabeling (green) was detected on TCs cell body, but it was stronger at Tps level, b Ca_V3.2 expression was detected only at cell body level. c Co-expression of Ca_V3.1-positive (green) and Ca_V3.2 (red) is presented on merged images. d Immunolabeling for T-type calcium channels in cell cultures from pregnant myometrium. Strong staining for Ca_V3.1 was found in the cytoplasm, adjacent to the nucleus and in the Tps of the TCs. e Ca_V3.2 immunolabeling was found throughout the cytoplasm of TCs and predominantly within Tps. f Merged images show co-expression pattern for Ca_V3.1 (green) and Ca_V3.2 (red). Note that both TCs and SMCs (cells with widened cell body without extensions) express Ca_V3.1 and Ca_V3.2. Nuclei were stained with DAPI. Bar 50 µm