(A) Locomotor behavior after Tdc2-Gal4-driven ablation of OA/TA neurons with UAS-grim, UAS-rpr, UAS-hid,rpr, or UAS-DTI. Experimental larvae showed significantly reduced performances compared to controls. (B) Rapid light avoidance behavior after specific ablation of photoreceptor neurons using lGMR-Gal4. Expression of Grim, Rpr, and Hid together with Rpr or DTI significantly reduced larval dark preferences compared to those of controls. Open box, experimental larvae; box with light shading, Gal4/+ larvae; box with dark shading, UAS/+ larvae. *P < 0.05; **P < 0.01; ***P < 0.001; n.s., P > 0.05. #, not significantly different from chance level.