Figure 4.

Locomotor behavior after optogenetic activation of OA/TA neurons. Expression of UAS-Pacα and UAS-bPac did not affect larval locomotor behavior as experimental larvae performed indistinguishably from control larvae during illumination. In contrast, blue light exposure specifically affected locomotor behavior of Tdc2-Gal4/UAS-ChR2-wt and Tdc2-Gal4/UAS-ChR2-XXL as they crawled significantly shorter distances compared to both controls. Remarkably, under red light conditions experimental larvae performed not significantly different from controls. Open box, experimental larvae; box with light shading, Gal4/+ larvae; box with dark shading, UAS/+ larvae. *P < 0.05; **P < 0.01; ***P < 0.001; n.s., P > 0.05.