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. 2014 Oct 29;199(1):25–37. doi: 10.1534/genetics.114.172023

Table 1. The genotypes, brief description of the mode of action, and the observed efficiencies of the effector genes used in this study.

Name Genotype Insertion chromosome(s) Reference Mechanism Efficiency in OA/TA neurons Efficiency in photoreceptor neurons
Cell ablation
UAS-reaper (rpr) w1118 P(UAS-rpr.C)27 1 Zhou et al. (1997) Binding to IAPs and thus inducing cell death ++ ++
UAS-grim w*; P{UAS-grim.N}2 2 Wing et al. (1998) Binding to IAPs and thus inducing cell death ++ ++
UAS-hid,rpr w, P[UAS-hid], P[UAS-rpr] 1 Zhou et al. (1997) Bindingto IAPs and thus inducing cell death +++ +++
UAS-DTI P{UAS-Cbβ\DT-A.I}14/TM3 3 Bellen et al. (1992) Inhibits protein synthesis ++ +++
Neuronal silencing
UAS-shibirets (shits) w; P{UAS-shits1.K}3 3 Kitamoto (2001) Temperature-sensitive dynamin impairs vesicle recycling +++ +++
UAS-TNTE w; P{UAS-TeTxLC.tnt}E2 2 Sweeney et al. (1995) Cleaves n-Syb and impairs vesicle docking − −
UAS-Kir2.1 w*; P{UAS-Hsap\KCNJ2.EGFP}1 2 Baines et al. (2001) Inward rectifying K+ channel prevents membrane depolarization +++ +++
UAS-ΔOrk y1 w*; P{UAS-Ork1.Δ-C}2 2 Nitabach et al. (2002) Outward rectifying K+ channel prevents membrane depolarization ++ +
Neuronal activation
UAS-TRPM8 yw; UAS-TRPM8 C4-D;UAS-TRPM8 C4-A, UAS-TRPM8 C1-A2) 2; 3 Peabody et al. (2009) Temperature-dependent cation channel opens in response to ∼12°–16° → cell depolarization − −
UAS-TRPA1 w; P{UAS-TRPA1(B).K}attP16 2 Rosenzweig et al. (2005) Temperature-dependent cation channel opens in response to ∼29° → cell depolarization +++ +++
UAS-Pacα P{UAS-Zzzz\PACα.SL} 3 Schröder-Lang et al. (2007) Eukaryotic photoactivatable adenylyl cyclase increasing intracellular cAMP − ND
UAS-bPac w*; P{UAS-bPAC.S} 2 Stierl et al. (2011) Bacterial photoactivatable adenylyl cyclase increasing intracellular cAMP − ND
UAS-ChR2 (a) P{UAS-ChR2.S}2; P{UAS-ChR2.S}3 (ChR2-wt) (a) 2; 3 (a) Nagel et al. (2003) Photoactivatable cation channel (470 nm) → cell depolarization (a) + ND
(b) UAS-ChR2-XXL/cyo:GFP (b) 2 (b) Dawydow et al. (2014) (b) +++
UAS-NaChBac y1 w*; P{UAS-NaChBac-EGFP}1/TM3, Sb1 3 Nitabach et al. (2006) Bacterial sodium channel increases sodium conductance → cell depolarization ++ ++
Gal4 lines
Tdc2-Gal4 w*; P{Tdc2-GAL4.C}2 2 Cole et al. (2005) Drives expression in octopaminergic/tyraminergic neurons
lGMR-Gal4 y1 w*; wgSp-1/CyO; P{longGMR-GAL4}3/TM2 3 Moses and Rubin (1991) Drives expression in photoreceptor cells