Figure 4.
Transcription and miR172-directed degradation of cly1 in immature spikes. Barley actin was used as the reference gene. (A) SV235, KNG, SV235 × KNG F1 hybrid. (B) SV235, SV230, SV235 × SV230 F1 hybrid. (1) lemma primordium stage, (2) stamen primordium stage, (3) awn primordium stage, (4) white anther stage, (5) green anther stage. Data shown as mean ± SE of at least three biological replicates. (C) miR172-directed cleavage of cly1 mRNA assayed by a modified 5′ RACE protocol. Vertical arrows indicate the inferred 5′ termini of miR172-guided cleavage, and the number above each arrow gives the proportion of clones showing these sites. cly1.b2 (SV235), cly1.b (KNG), cly1.c (SV230), Cly1.a (AZ).