Table 3. DNA methylation at the key –245 cytosine site in immature spikes and leaves.
| No. of clones | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cultivar | Allele | Stamen primodium stage | Green anther stage | Leaf | miR172-assisted mRNA cleavage | Vascular tissue | ||||||
| Nonmethylated | Methylated | % | Nonmethylated | Methylated | % | Nonmethylated | Methylated | % | ||||
| SV235 | cly1.b2 | 4 | 12 | 75 | 12 | 4 | 25 | 16 | 3 | 19 | Not cleaved | Partially developed |
| KNG | cly1.b | 12 | 4 | 25 | 16 | 0 | 0 | 16 | 1 | 6 | Not cleaved | Not developed |
| SV2308a | cly1.c | — | — | — | — | — | — | — | — | — | Not cleaved | Not developed |
| GP | cly1.b | 16 | 0 | 0 | 16 | 0 | 0 | n.t., not tested | n.t. | n.t. | Not developed | |
| AZ | Cly1.a | 14 | 2 | 13 | 16 | 0 | 0 | n.t. | n.t. | Cleaved (Nair et al. 2010) | Developed | |
| Morex | Cly1.a | 12 | 4 | 25 | 15 | 1 | 6 | n.t. | n.t. | n.t. | Developed | |
a
Sequence variance (C/T) replaced CG site in SV230.