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. Author manuscript; available in PMC: 2015 Jan 8.

Published in final edited form as: Cancer Res. 2010 Oct 19;70(21):8736–8747. doi: 10.1158/0008-5472.CAN-10-0902

(A) Growth inhibition in human melanoma cell lines treated with AZD6244 for 48 h. X-axis, concentration of AZD6244 (nM); Y-axis, % growth inhibition. Groups of cells with high (“1”), moderate (“2”), and low (“3”) sensitivity are indicated. Cells with data points connected by dotted lines are wild-type for Braf, all other cell lines have Braf mutations. Data points are the average of 3 replicates; error bars, standard deviation. (B) Cell cycle analysis of WM35 cells (Group 1) after 72 h treatment AZD6244. X-axis, concentration of AZD6244 (nM); Y-axis, % of cell population. The Sub-G1 (dead cells), G1, S, and G2/M phases of the cell cycle are indicated by black, dark grey, light grey and white bars respectively. Each bar is the average of two replicates; error bars, standard deviation. (C) Cell cycle analysis of SKMEL5 cells (Group 2). (D) Cell cycle analysis of HS294T cells (Group 3).

(A) Growth inhibition in human melanoma cell lines treated with AZD6244 for 48 h. X-axis, concentration of AZD6244 (nM); Y-axis, % growth inhibition. Groups of cells with high (“1”), moderate (“2”), and low (“3”) sensitivity are indicated. Cells with data points connected by dotted lines are wild-type for Braf, all other cell lines have Braf mutations. Data points are the average of 3 replicates; error bars, standard deviation. (B) Cell cycle analysis of WM35 cells (Group 1) after 72 h treatment AZD6244. X-axis, concentration of AZD6244 (nM); Y-axis, % of cell population. The Sub-G1 (dead cells), G1, S, and G2/M phases of the cell cycle are indicated by black, dark grey, light grey and white bars respectively. Each bar is the average of two replicates; error bars, standard deviation. (C) Cell cycle analysis of SKMEL5 cells (Group 2). (D) Cell cycle analysis of HS294T cells (Group 3).