Table 5. Predicted Peptides for HCV E1 and E2 Conserved in Genotype 5a.
| Position a | Peptides | Length | Molecular Weight | Theoretical PI | Extinction Coefficient (/cm M) | Instability Index | Alphatic index | GRAVY | Composition of Hydrophobic AA’s, %b | N-linked Glycosylation C c | N-linked Phosphorylation |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 201 | YHTNDCPNSSI | 14 | 1611.7 | 5.08 | 2980 | 17.36 | 69.29 | -0.343 | 21.4 | + | - |
| 262 | VDYLAGGAA | 9 | 835.9 | 3.80 | 1490 | -3.53 | 108.89 | 0.867 | 22.2 | - | - |
| 304 | CNCSIYSGH | 9 | 983 | 6.72 | 1615 | 5.69 | 43.33 | -0.056 | 11.1 | ++ | - |
| 314 | TGHRMAWDMMMNWSPT | 16 | 1952.2 | 6.41 | 11000 | 29.16 | -0.706 | 6.25 | 37.5 | - | - |
| 352 | HWGVLFAAAY | 10 | 1134.3 | 6.74 | 6990 | -4.25 | 98 | 1.040 | 40 | - | - |
| 562 | VKTCGAPPC | 9 | 875 | 8.03 | 125 | 30.68 | 43.33 | 0.311 | 11.1% | - | - |
| 585 | TDCFRKHP | 8 | 1003.1 | 7.92 | 0 | 5.15 | 0 | -1.512 | 12.5 | - | - |
| 645 | ACNWTRGERCDL | 12 | 1423.5 | 6.1 | 5625 | 27.31 | 40.83 | -0.908 | 16.7 | + | - |
| 664 | LSPLLHTTTQ | 10 | 1110.2 | 6.74 | - | 37.86 | 117 | 0.020 | 30% | - | - |
| 675 | AILPCSFTPTPALSTGLIHLHQNIVDTQ | 28 | 2988.4 | 5.97 | 0 | 28.19 | 115 | 0.421 | 32.1 | - | - |
| 725 | FLLLADAR | 8 | 918.1 | 5.84 | - | -1.86 | 171.25 | 1.225 | 50 | - | - |
a Numbering is based on the M62321 full-length sequence.
b list of hydrophobic amino acids (Leu, Val, Ile, Met, Phe and Trp).
c Glycosylation probability is shown by +++ (probability > 70%), ++ (probability between 60 and 70%), + (probability between 50 and 60%), and - (not present).