Table 2.
Iron content of wild type and mutants of SeADO measured by ICP-OES assay
| Protein | Expression conditions | Number of iron atom per ADO molecule | Percentage of iron content compared with wild type 3 | ||
|---|---|---|---|---|---|
| LB medium | TB medium | LB medium + Fe | |||
| Wild type 1 | √ | 0.28 ± 0.004 | 17.7% ± 0.25% | ||
| Wild type 2 | √ | 0.67 ± 0.02 | 42.4% ± 1.27% | ||
| Wild type 3 | √ | 1.58 ± 0.09 | 100% ± 5.7% | ||
| E144A | √ | 1.58 ± 0.06 | 99.9% ± 3.81% | ||
| Y122F | √ | 1.69 ± 0.12 | 107.38% ± 7.7% | ||
| F86YF87Y | √ | 1.30 ± 0.26 | 82.29% ± 16.78% | ||
| A129insI | √ | 1.60 ± 0.29 | 101.32% ± 18.17% | ||
| A129insIF | √ | 1.58 ± 0.03 | 100.25% ± 1.63% | ||
| L146S | √ | 1.48 ± 0.01 | 93.96% ± 0.76% | ||
| R148A | √ | 1.53 ± 0.08 | 96.64% ± 5.17% | ||
| N149A | √ | 1.35 ± 0.23 | 85.50% ± 14.71% | ||