Figure 4.

Ey PD and HD physically interact with each other. (A) Diagram of Ey WT and deletion constructs and GST-fusion Ey proteins. PD and HD are shown in yellow and blue filled boxes, respectively. (B) GST-pull down experiments. ³⁵S-labelled in vitro translated proteins were marked with *. A 50% or 25% input of the ³⁵S labeled proteins was shown on the right panel. ³⁵S-labeled WT Ey binds and pulled down any GST-fusion Ey deletion proteins. Deletion of both PD and HD (∆PD, ∆HD*) abolished interaction and was not pulled down by any GST-fusion proteins. HD* or PD* was pulled down by GST-PD or GST-HD reciprocally. Heterologous domain interaction increased pulled down