Figure 2. Cis-ligand represses ligand-independent Notch activity in the follicle cells and imaginal discs.
DlrevF10 mutant MARCM germline/follicle cell clones co-expressing NotchRNAi show prolonged Cut expression (A). Su(H)47 MARCM mutant germline/follicle cell clones co-expressing DlRNAi show failure to enter the endocycle (B). Germline clones are shown by late Cut expression in wild-type follicle cells (A, B, see arrowheads). See Figure 2—figure supplement 1A for control DlRNAi-induced germline follicle cell clones. Notch Responsive Element-green fluorescent protein (NRE-GFP) is upregulated beginning from stage 2 (C) and through later stages (D) in DlRevF10 germline and follicle cell clones. NRE-GFP is also upregulated cell-autonomously in DlRevF10SerRx82 mutant clones in eye (E) and wing (F) imaginal discs. Scale bars represent 20 μm.
Figure 2—figure supplement 1. Control experiments relating to Figure 2.
The Dl-/Dl- phenotype can also be recapitulated using DlRNAi, which knocks down Dl in both the germline and soma using the FLP-out method (A and B). See the arrowhead in (B) for wild-type (WT) Dl staining. Again, germline clones are evidenced by aberrant Cut expression in WT follicle cells. MARCM-induced clones expressing only NotchRNAi show late Cut expression (C). Su(H)47 mutant clones created using the MARCM system also show late Cut staining and smaller nuclei (D). Scale bars represent 20 μm.