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. 2015 Jan 8;5:7688. doi: 10.1038/srep07688

Figure 2. Itk tunes the expression of Eomes and development of MPECS during infection.

Figure 2

Naïve WT or Itk−/− T cells were transferred separately into WT mice, which were infected with L. monocytogenes. Mice were bled on the indicated days and cells analyzed as indicated. (A) Cell number. (B) Bacterial numbers in liver determined from mice infected for day 2 or day 7, and. n = 4, 3 independent experiments, no bacteria detected. (C) Percent SLEC and MPEC. (D) Percent T-bet and Eomes. *p < 0.05, n = 3–8 mice, 3 independent experiments. (E) Flow cytometric analysis of the expression of CD127 (left panels) or Eomes (right panels) in representative mice. (F) Naïve WT or Itk−/− T cells were stimulated with 20 μg/ml OVA plus antigen presenting cells over 5 days. Cells were analyzed for expression of IRF4 by flow cytometry. n = 3. (G) IRF4 expression was determined by flow cytometry in WT or Itk−/− OTI/Rag−/− T cells following transfer into WT mice and infection with L. monocytogenes for 5 days. n = 3–8 mice, 3 independent experiments. All error bars indicate the value of +/−SEM.