Fig. 1.

Purification of atractylochromene as an inhibitor of TOP-Flash activity. The rhizomes of A. macrocephala were extracted with ethyl acetate as described in materials and methods. After treatment of Wnt3a CM and test samples (column fractions of ethyl acetate extracts) to TOPFlash reporter stable HEK-293 cells for 15 h, TOPFlash reporter activity was determined by measuring firefly luciferase activity (A). Structure of atractylochromene (B). Data shows the relative luciferase activity by mean ± S.D. of three experiments. The asterisks indicate a significant difference from the Wnt3a CM treated vehicle group (*p<0.01).