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Atractylochromene inhibits TOPFlash activity. After treatment of Wnt3a CM (A) or LiCl (20 mM) (B), and atractylochromene (20 μg/ml) to the TOPFlash reporter stable HEK-293 cells for 15 h, TOPFlash reporter activity was determined by measuring firefly luciferase activity. Data shows the relative luciferase activity by mean ± S.D. of three experiments. The asterisks indicate a significant difference from the Wnt3a CM treated vehicle group (*p<0.01).
