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. 2015 Jan 7;6:5935. doi: 10.1038/ncomms6935

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(a) Electron density map of the thiohemiacetal intermediate obtained from E268A-NAD⁺ crystal soaked with 2-HMS for 30 min. (b) Electron density map of the thioacyl intermediate obtained from E268A-NAD⁺ crystal soaked with 2-HMS for 180 min. The 2F_o−F_c electron density map for ligands and Cys302 is contoured to 1.0 σ and shown as a blue mesh. The omit map for ligands and Cys302 is contoured to 2.0 σ and shown as a gray mesh.