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Alizarin Red Staining for calcium deposit in hMSC cultures treated with growth media (MG), growth media with 200 μM citrate supplement (MG200), osteogenic media (OG), osteogenic media with 200 μM citrate supplement (OG200) at the 7th, 14th, and 21st day. Citrate in markedly enhanced calcium deposit formation of osteogenic differentiated hMSCs (Alizarin Red Staining, 40X).
