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. 2014 Sep 4;15(1):757. doi: 10.1186/1471-2164-15-757

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© Rengaraj et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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qPCR analysis of piRNAs and piRNA-linked non-coding gene. qPCR quantitative expression analysis of three repeat sequence-linked putative piRNAs that were upregulated in PGCs, along with CR1. cDNA templates used for the amplification of piRNAs and genes were prepared separately, and amplified using the appropriate piRNA- and gene-specific primers. The expression of piRNAs and CR1 were normalized against that of snoRNA and GAPDH, respectively. Bars indicate the SEM of triplicate analyses.