Table 5.
List of top 20 genic sequences originated piRNAs that upregulated in PGCs
| piRNA seq. ID | Sequence length | Sequences | Origin | Genomic distribution | Associated gene | PGCs | Stage X | GSCs | CEFs | Fold change |
|---|---|---|---|---|---|---|---|---|---|---|
| (RPKM) | (RPKM) | (RPKM) | (RPKM) | |||||||
| ISG_2943457* | 25 | GACAGACAGAGCTGCCCCTGAGCCT | Intron_antisense | NM_001146136_intr_4 | CHIR-B5 | 55.72 | 0 | 0.87 | 0 | 191.65 |
| ISG_1259042* | 27 | AGACTGAAGATGTGCACCTGACGCCAG | Intron_sense | NM_001146141_intr_4 | CHIR-AB1 | 49.62 | 0 | 0.76 | 0 | 195.06 |
| ISG_2785619* | 26 | TTCTGCATGTTGCTCTCTGTCAGCTG | Intron_sense | NM_001030561_intr_5 | PLLP | 42.92 | 0 | 0.33 | 0 | 393.64 |
| ISG_2237691 | 25 | AGACTGAAGATCTGCACCTGACACC | Intron_sense | NM_001146136_intr_6 | CHIR-B5 | 35.6 | 0 | 0.87 | 0 | 122.45 |
| ISG_2022559* | 27 | TGTTTACTGACTGAGCTACTTTTCCCC | Intron_sense | NM_205163_intr_15 | MYO1A | 33.41 | 0 | 0.11 | 0 | 919.25 |
| ISG_2633063* | 26 | AGGGTACTGAGACATCTTGGAGACAA | Intron_antisense | NM_204716_intr_4 | SLC6A2 | 19.87 | 0 | 0.33 | 0 | 182.28 |
| ISG_1354003* | 21 | TTTCCAAGGACCAGTAGCGCT | Intron_sense | NM_205429_intr_1 | 17.5 | 18.29 | 0 | 0.55 | 0 | 100.65 |
| ISG_2087909* | 26 | TCTCAAAGGATTCCGCATCGTCGACG | Exon_antisense | NM_001030702_exon_1 | RAP2B | 11.95 | 0 | 0 | 0 | 1194.81 |
| ISG_3065006* | 28 | AAGGACCCAAATGGTAGCAGAGGCCATG | Intron_antisense | NM_205186_intr_18 | LRP8 | 9.51 | 0 | 0.11 | 0 | 261.68 |
| ISG_3356981* | 25 | AATGCTGAGAACTAAGGATGCCTCC | Intron_sense | NM_205098_intr_2 | VDR | 7.92 | 0 | 0 | 0 | 792.48 |
| ISG_3294350* | 30 | CAGGCTGTGACCCTGGAATTCCACTACACT | Intron_sense | NM_001013397_intr_72 | MYH1E | 7.44 | 0 | 0.11 | 0 | 204.65 |
| ISG_2645108* | 27 | GAGTGTGAGAAGGGCTTTGTGCAGAGC | Exon_antisense | NM_001030695_exon_5 | ZNF302 | 5.24 | 0 | 0 | 0 | 524.26 |
| ISG_2675670* | 23 | CGGTGTGGGACGAGAAGGAGAAC | Exon_sense | NM_204729_exon_2 | RGN | 4.88 | 0 | 0 | 0 | 487.68 |
| ISG_3080707* | 27 | CTGTGAGTGTGTGAGTGCGGCGGCGCG | Intron_antisense | NM_204952_intr_1 | FOXD2 | 4.27 | 0 | 0.11 | 0 | 117.42 |
| ISG_3280151* | 24 | AAGGACCTCTGAGAATTGCTTTCT | Exon_sense | NM_001030884_exon_12 | RASSF2 | 2.32 | 0 | 0 | 0 | 231.65 |
| ISG_1621199 | 27 | CAGAAGAGAAGCTGAACACAGGGTGTC | Intron_antisense | NM_001031121_intr_7 | VAMP7 | 1.95 | 0 | 0 | 0 | 195.07 |
| ISG_882291 | 28 | TTAAAGATATTTGGCTGCCTGGCTCGCC | Intron_sense | NM_001081502_intr_4 | NLGN1 | 1.95 | 0 | 0 | 0 | 195.07 |
| ISG_2363541 | 26 | AAGGACACCGAGGCTCTGCGTGCTGA | Exon_sense | NM_205525_exon_2 | APOA1 | 1.83 | 0 | 0 | 0 | 182.88 |
| ISG_2033456 | 26 | TCGGCTCGGCTCGGCTCGGCTCGGCT | Exon_antisense | NM_001006501_exon_1 | ADK | 1.1 | 0 | 0 | 0 | 109.73 |
| ISG_950928 | 27 | ATCTGCGTTTAAAGCTCTTTGCACACT | Intron_antisense | NM_001030561_intr_5 | PLLP | 1.1 | 0 | 0 | 0 | 109.73 |
CHIR-B5: immunoglobulin-like receptor CHIR-B5; CHIR-AB1: immunoglobulin-like receptor CHIR-AB1; PLLP: plasmolipin; MYO1A: myosin IA; SLC6A2: solute carrier family 6, member 2; 17.5: lectin-like protein, type II transmembrane protein (17.5); RAP2B: RAP2B, member of RAS oncogene family; LRP8: low density lipoprotein receptor-related protein 8, apolipoprotein e receptor; VDR: vitamin D3 receptor; MYH1E: myosin, heavy chain 1E, skeletal muscle; ZNF302: zinc finger protein 302; RGN: regucalcin; FOXD2: forkhead box D2; RASSF2: Ras association (RalGDS/AF-6) domain family member 2; VAMP7: vesicle-associated membrane protein 7; NLGN1: neuroligin 1; APOA1: apolipoprotein A-I; ADK: adenosine kinase. *piRNAs were examined by qPCR along with their associated genes.