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Apoptotic activity evidenced by cleaved caspase-3 antibody. Ungrafted controls are shown in (A,E,I). Slow-frozen grafted tissue (group A) is shown in (B,F,J). Slow-frozen tissue supplemented with NAC (group B) is shown in (C,G,K). Slow-frozen tissue supplemented with testosterone (group C) is shown in (D,H,L). Immature testicular tissue is from boys of 2 (A–D), 8 (E–H), and 14 years of age (I–L). Scale bar: 50 μm.
