Figure 6.

Differential scanning calorimetry of Lys-acetyl protein charge ladders of ALS-variant apo-SOD1 and Zn²⁺-loaded SOD1. (A) Endothermic transitions of acetylated and non-acetylated G85R apo-SOD1 and G85R Zn₂-SOD1. The T_m value for each protein is listed next to each endothermic peak. The metal content was confirmed with ICP-MS. The average number of acetylated lysines in each ladder (Ac(∼N)) are listed per dimer. (B–D) Same experiments as in part A, but for D90A, G93R, and E100K apo-SOD1 and Zn₄-SOD1 proteins.