Table 2.

“Charge Gap” Values (ΔZ_ALS:WT) between WT and ALS-variant SOD1 at Different Metalation States as Measured by Capillary Electrophoresis at pH 7.4, 20°C

	ΔZALS:WT
SODa	D90A	G85R	G93R	E100K
apoS-S	2.82 ± 0.08	3.42 ± 0.08	3.22 ± 0.09	3.77 ± 0.08
Zn1(b)	2.00 ± 0.23	1.74 ± 0.23	1.87 ± 0.23	2.70 ± 0.23
Zn2(e)	2.16 ± 0.31	2.17 ± 0.32	2.53 ± 0.30	2.81 ± 0.32
Zn3(i)	2.53 ± 0.11	N/A	2.35 ± 0.11	2.72 ± 0.11
Zn4(k)	2.42 ± 0.12	N/A	2.40 ± 0.12	2.82 ± 0.12

^aThe labels “a”, “e”, etc., correspond to labels of electrophoretically unique states in Figure 2.