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Functional properties of YjcS. (A) SDS hydrolysis of YjcS on minimal media agarose plates. Samples of wild-type YjcS (Y) and the double mutant D184N-H185A (M) were diluted sequentially by fivefold and plated at positions 1–5. The upper one is cultured at 37°C for 2 days and the lower one was cultured for 1 week. (B) The effects of Ca2+ and mutant on the enzyme activity of YjcS-mediated SDS hydrolysis in vitro. The released protons are calculated based on the standard curve of HCl.
