Figure 2.

BLOC-1 presynaptically regulates spontaneous neurotransmission at the Drosophila NMJ. A–E, Representative EJP and mEJP traces showing Blos1-null (A, red, Blos1^EY) and Blos1 heterozygote (B, blue, Blos1^EY/+) increase mEJP but not EJP amplitude compared with control (Canton S, black). Presynaptic expression of UAS-Blos1 in a single-copy Blos1 loss (C, green) or single-copy loss of Blos1 in trans with the dysb¹ mutation (D, red) rescues mEJP amplitude compared withBlos1 heterozygote (C and D, blue). Presynaptic expression of dysb in Blos1^EY;dysb¹ transheterozygote (E, green) increases mEJP amplitude compared with the transheterozygote (E, red). No changes in EJP amplitude or mEJP frequency were observed in any of the genotypes. Asterisk denotes that there are no differences between w1118 and Canton S control strains (F). G–R, Event size plotted against frequency of event for BLOC-1 loss-of-function allelic combinations. Shift in the curve to the right indicates larger events in that genotype. All comparisons in F were performed with one-way ANOVA followed by Bonferroni's multiple-comparison test. Comparisons were performed by Kolmogorov–Smirnov test. F–R, Data were obtained from 7 to 11 animals per genotype. G–R, Plots graph between 992 and 2880 randomly selected mEJP events.