Figure 4.

Normal synaptic morphology requires BLOC-1 function. A–I, Representative images of HRP immunofluorescence at the 6/7 synapse in Drosophila for indicated genotypes. J, Significant increases in the number of synaptic boutons in BLOS1-null animals (Blos1^EY, Blos1^ex65) and animals carrying single-copy loss of BLOS1 (Blos1^EY/+, Blos1^ex65/+, Blos1^EY/rv, and Blos1^ex65/rv), but no significant changes in synaptic bouton numbers in Blos1^EY/+;dysb^1/+ or Blos1^ex65/+;dysb^1/+ transheterozygotes. K, Muscle area across all genotypes was unaffected. All comparisons were performed with one-way ANOVA followed by Dunnett's multiple-comparison test. Data were obtained from 10 to 14 animals per genotype. *p between 0.0019 and 0.045 and NS indicates nonsignificant p values.