Figure 6.

BLOC-1 gene dosage regulates synaptic vesicle pool properties. Animals were stimulated at low frequency(3 Hz; A–C) and high frequency (10 Hz; D–F) in the presence of 1 μm bafilomycin A1. Inset shows stimulation to 50% depletion compared with response at stimulation 0. No changes in rate of vesicle pool depletion were observed across genotypes at low frequency (A–C). At high frequency, no changes were observed in vesicle depletion rate in dysb¹ (D, red) or Blos1^EY/+ (D, blue) compared with control (D, w1118, black). Blos1^EY (E, blue) and Blos1^EY/+;dysb^1/+ transheterozygote (F, blue) both displayed significantly faster vesicle depletion compared with the Blos1^EY/+ (E, F, black). All comparisons in F and G were performed with one-way ANOVA followed by Fisher's multiple comparison. Data were obtained from 6 to 9 animals per genotype.