Figure 7.

Identification of chromatin modifying complex recruiting H3K9 methyltransferases. a, A MEME-ChIP analysis was performed to identify the transcription factor binding motifs enriched within the genomic locations characterized by H3K9me3 unique peak sequences in OLs. Top, Consensus motif for each transcription factor. Bottom, Query motifs. b, Western blot analysis to identify the chromatin modifying complex and transcription factors during OPC differentiation using coimmunoprecipitation. c, ChIP of samples isolated from progenitors (OPCs) and differentiating OLs and immunoprecipitated with antibodies against H3K9me3, H3K27me3, YY1, and SOX10. Diagram represents the H3K9me3 peaks identified by ChIP-seq in two genes: one encoding the potassium channel subunit Kcnb1 and the other one encoding a neuronal transcription factor (Lhx1). The relative position of the YY1 (pink boxes) and SOX10 binding sites (green boxes) and the regions of DNA (roman numerals) amplified by specific primer pairs are indicated. Arrows mark the TSS. n = 2 independent cultures. *p < 0.05. **p < 0.01. ***p < 0.001 (unpaired Student's t test).