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. 2015 Jan 7;35(1):422–437. doi: 10.1523/JNEUROSCI.1509-14.2015

Figure 6.

Figure 6.

Physiological characteristics of functionally restored GlyRs from independent domains. A, Ligand-binding experiments were performed using membrane preparations of transfected HEK293 with truncated GlyR α1 variants R316X and W68C/R316X together with the tail construct (transfected ratio was 1: 5 of truncated α1: C-terminal domain). B, Whole-cell recordings from transfected HEK293 cells with rescues R1, R2, and R3 coexpressing a truncated GlyR α1 variant together with the tail domain iD-TM4-C: R1 (R316X + iD-TM-C), R2 (W68C/R316X + iD-TM4-C), and R3 (W68C + R316X + iD-TM4-C). Glycine was applied at 1 mm. Representative traces are shown above the bars of the corresponding rescue condition. Imax values result from at least four independent experiments.