Table 1.
Functional properties of GlyRα1 variants
| Localization | GlyR variant | Imax 1 mM gly (nA) | n | Imax 10 mM gly (nA) | n |
|---|---|---|---|---|---|
| Loop B | WT | 3.03 ± 0.5 | 25 | 2.2 ± 0.4 | 20 |
| G160R | 3.2 ± 0.7 | 10 | 5.3 ± 1.2 | 7 | |
| T162M | 4.8 ± 0.6 | 10 | 1.4 ± 0.2 | 11 | |
| Loop D/β2–3 | W68C | 0 ± 0 | 9 | 0 ± 0 | 10 |
| D70N | 0.02 ± 0.004* | 6/14* | 0 ± 0 | 10 | |
| R72H | 0 ± 0 | 10 | 0 ± 0 | 10 | |
| TM3–4 loop | R316X | 0 ± 0 | 10 | 0 ± 0 | 10 |
| TM3–4 loop, TM4, C-terminus | iD-TM4-C | 0 ± 0 | 10 | 0 ± 0 | 10 |
| TM3–4 loop | R316X + iD-TM4-C (R1) | 0.45 ± 0.12 | 16 | 0.7 ± 0.2 | 11 |
| Loop D/TM3–4 loop | W68C/R316X | 0 ± 0 | 10 | 0 ± 0 | 10 |
| Loop D/TM3–4 loop | W68C/R316X + iD-TM4-C (R2) | 0 ± 0 | 21 | 0 ± 0 | 10 |
| Loop D/TM3–4 loop | W68C + R316X + iD-TM4-C (R3) | 0.5 ± 0.13 | 13 | 0.12 ± 0.02 | 11 |
| TM4 | W407R | 0 ± 0 | 10 | 0 ± 0 | 10 |
| β2–3/TM4 | D70N/W407R | 0 ± 0 | 10 | 0 ± 0 | 16 |
| Localization | GlyR variant | FLEX station experiments |
|||||
|---|---|---|---|---|---|---|---|
| EC50 gly (μm)FLEX | n | EC50 tau (μm)FLEX | n | EC50 β-ala (μm)FLEX | n | ||
| Loop B | WT | 58 ± 2.5 | 24 | 443 ± 62 | 6 | 120 ± 11 | 6 |
| G160R | 43 ± 3 | 18 | 85 ± 5 | 6 | 43 ± 2 | 6 | |
| T162M | 409 ± 10 | 14 | >162000 | 3 | 480 ± 91 | 6 | |
| Loop D/β2–3 | W68C | NB | ND | ND | |||
| D70N | NB | ND | ND | ||||
| R72H | NB | ND | ND | ||||
| Localization | GlyR variant | Whole-cell recordings |
|||||
|---|---|---|---|---|---|---|---|
| EC50 gly (μm) | n | EC50 tau (μm) | n | EC50 β-ala (μm) | n | ||
| Loop B | WT | 26 ± 3 | 6 | 271 ± 22 | 6 | 63 ± 12 | 6 |
| G160R | 90 ± 8 | 6 | 55 ± 6 | 6 | 120 ± 25 | 6 | |
| T162M | ND | ND | ND | ||||
| Loop D/β2–3 | W68C | NF | NF | NF | |||
| D70N | NF | NF | NF | ||||
| R72H | NF | NF | NF | ||||
EC50 values represent mean values ± SEM. n, Number of measured cells out of different transfections; NB, nonbinding; ND, not determined; NF, nonfunctional.
* Calculated from responded cells only out of n cells recorded.