Table 2.
Ligand binding properties of GlyRα1 variants
| Localization | GlyR variant | Bmax (pmol × mg−1) | KD (nm) | n |
|---|---|---|---|---|
| Loop B | WT | 15367 ± 4025 | 115 ± 66 | 9 |
| G160R | 7650 ± 939 | 136 ± 12 | 3 | |
| T162M | NB | — | 4 | |
| Loop D/β2–3 | W68C | NB | — | 3 |
| D70N | NB | — | 3 | |
| R72H | NB | — | 3 | |
| TM3–4 loop | R316X | NB | — | 4 |
| TM3–4 loop, TM4, C-terminus | iD-TM4-C | NB | — | 3 |
| TM3–4 loop | R316X + iD-TM4-C | 533 ± 257 | 92 ± 12 | 4 |
| Loop D/TM3–4 loop | W68C/R316X | NB | — | 3 |
| Loop D/TM3–4 loop | W68C/R316X + iD-TM4-C | NB | — | 3 |
| TM4 | W407R | NB | — | 4 |
| β2–3/TM4 | D70N/W407R | NB | — | 3 |
n, Number of independent experiments; concentrations of 3(H)strychnine 0.1–200 nm were used. Bmax and KD values represent mean values ± SEM. NB, Nonbinding.