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Estimation of secondary structure content from CD spectra of refolded GlyR ECDs
| Variant | α-Helix (%) | β-Strand (%) | Turns (%) | Unordered (%) | Sum (%) |
|---|---|---|---|---|---|
| WT | 22.5 | 23.9 | 12.9 | 35.1 | 94.0 |
| W68C | 30.4 | 17.4 | 12.5 | 33.8 | 94.1 |
| D70N | 23.8 | 22.9 | 12.5 | 34.5 | 93.7 |
| R72H | 23.5 | 23.1 | 12.5 | 34.4 | 93.6 |
| G160R | 23.1 | 25.5 | 12.5 | 34.7 | 95.8 |
| T162M | 22.5 | 25.0 | 12.5 | 35.0 | 95.0 |
| SD (%) | 11.9 | 11.1 | 4.15 | 10.3 |
Analysis was performed using the method of Raussens et al. (2003, 2006).
