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. 2014 Jun 16;3(4):497–512. doi: 10.1002/mbo3.183

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© 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The effect of acetyl∼P availability on the expression of essential components for σ^N-dependent regulation of GDAR and the LEE. (A) Expression levels of genes in order from bottom to top: gadE, ler, flhD, and fliZ plotted for wild-type and derivative strains grown in MOPS with glucose (black), glycerol (white), or glycerol and acetate (gray). (B) gadE and ler expression levels plotted for wild-type (black), ΔntrC (white), ΔrpoN (stippled), ΔackA (gray), and ackA complement strain ΔackApRAM-8 (hatched). Plots that differ in lowercase letter for each strain (A) or gene (B) differ significantly by Tukey's HSD following a significant F-test (n ≥ 3, P < 0.05). Error bars denote standard deviation. GDAR, glutamate-dependent acid resistance; LEE, locus of enterocyte effacement.