Table 1.
Strains and plasmids used in this study.
| Strain/Plasmid | Relevant characteristics | Source/Reference |
|---|---|---|
| Strain name | ||
| DH5α | Vector propagation, recA1 endA1 | |
| BL-21 | Miroux and Walker (1996) | |
| TW14359 | WT O157:H7 2006 outbreak, western US (NC_013008.1) | Manning et al. (2008) |
| EcRPF-6 | TW14359ΔrpoN | Mitra et al. (2012) |
| EcRAM-26 | TW14359ΔntrC | Mitra et al. (2012) |
| EcRAM-43 | TW14359ΔrpoNGln+, suppressor mutant for Gln auxotrophy | This study |
| EcRAM-45 | EcRAM-43ΔglnA | This study |
| EcRAM-47 | TW14359crl::kan KanR | This study |
| EcRAM-49 | TW14359ΔfliZ | This study |
| EcRAM-51 | EcRFP-6 pRAM-3 AmpR | This study |
| EcRAM-52 | EcRAM 26 pRAM-3 AmpR | This study |
| EcRAM-53 | EcRAM 49 pRAM-3 AmpR | This study |
| EcRAM-58 | TW14359ΔflhDC | This study |
| EcRAM-59 | EcRAM 58 pRAM-4 AmpR | This study |
| EcRAM-60 | EcRAM 58 pRAM-5 AmpR | This study |
| EcRAM-61 | EcRAM 58 pRAM-6 AmpR | This study |
| EcRAM-63 | TW14359ΔackA | This study |
| EcRAM-66 | TW14359ΔfliZpRAM-8 | This study |
| EcRAM-68 | TW14359ΔackApRAM-9 | This study |
| Plasmid name | ||
| pACYC177 | Low-copy cloning vector, AmpR KanR P15A | Chang and Cohen (1978) |
| pET-24d | IPTG-inducible His-tagging vector, KanR | Novagen |
| pBAD22 | Mid-copy arabinose-inducible cloning vector, AmpR | Guzman et al. (1995) |
| pSC-B | High-copy cloning vector, AmpR KanR | StrataClone |
| pBAD-TA | Mid-copy arabinose-inducible cloning vector, AmpR | Invitrogen |
| pRAM-1 | rpoN::pACYC177, AmpR KanS | Mitra et al. (2012) |
| pRAM-3 | flhDC::pBAD22, AmpR | This study |
| pRAM-4 | flhDC::pACYC177 positions +948 to −1994 relative to start codon | This study |
| pRAM-5 | flhDC::pACYC177 positions +948 to −825 relative to start codon | This study |
| pRAM-6 | flhDC::pACYC177 positions +948 to −728 relative to start codon | This study |
| pRAM-7 | ntrC::pET-24d containing ORF, KanR | This study |
| pRAM-8 | fliZ::pBAD, AmpR | This study |
| pRAM-9 | ackA::pSC-B, AmpR KanR | This study |