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. 2015 Jan 1;31(1):98–106. doi: 10.1089/aid.2014.0121

FIG. 2.

FIG. 2.

Luciferase assays of TALE-VP64-mediated HIV-1 5′-LTR activity. (A) Transient reporter assays comparing the activation potential of various designer TALE-VP64 transcription factors. HEK-293 cells were transfected with an HIV LTR-luciferase reporter plasmid and a plasmid expressing the chimeric protein indicated; the empty vector pcDNA3.1(-) was used as a control. Then, 48 h posttransfection, the relative luciferase activity was measured. The data represent the mean±standard deviation of three independent experiments. *p<0.05, **p<0.01, ***p<0.001; paired t test. (B) Synergistic activation of HIV-1 by TALE1-VP64 and Tat. HEK-293 cells were transfected with the TALE1-VP64 expression plasmid in the presence or absence of Tat; the empty vector pcDNA3.1(-) was used as a control. Then, 48 h posttransfection, the relative luciferase activity was measured. The data represent the mean±standard deviation of three independent experiments.