FIG. 6.

Effects of TALE1-VP64 on cell proliferation and cell cycle distribution in human peripheral blood mononuclear cells (PBMCs). (A) PBMCs were transfected with pcDNA3.1(-) or TALE1-VP64. Then, 48 h posttransfection, the cell viability was measured using a CCK-8 kit (Dojindo). The cell viability of the transfected groups was normalized to that of the untransfected group. The data are presented as the means±standard deviations of three independent experiments. (B) PBMCs were transfected with pcDNA3.1(-) or TALE1-VP64. Then, 48 h posttransfection, the distribution of the cells in each phase of the cell cycle was determined based on their DNA content via propidium iodide staining and FACS analysis.