Overview	Use denaturing agarose gel with formaldehyde to separate RNA based on the size.
Duration	5 hrs
1.1	Assemble agarose gel rigs and make denaturing RNA gel solution.
1.2	Pour the RNA gel in hood
1.3	After gel solidify, equilibrate gel with running buffer for at least 30 min before running.
1.4	Mix 15 μg RNA sample with equal volume of 2× RNA loading buffer. Dilute 3 μg Millennium RNA Markers in same volume of 2× RNA loading buffer. Incubate @ 65 °C in the heating blocking for 12~15 min and put samples on ice immediately afterwards.
1.5	Load all the samples to equilibrated gel and leave space between first sample and RNA marker.
1.6	Run gel at 125 V for about 3 hrs
Tip	All the sample volume should not exceed the well volume of the gel comb. If RNA sample is too diluted, salt precipitate the RNA and resuspend with less H2O.