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. Author manuscript; available in PMC: 2015 Jan 8.
Published in final edited form as: Radiat Res. 2012 Dec 5;179(1):101–112. doi: 10.1667/RR3120.1

FIG. 6.

FIG. 6

Radiation and radiation combined injury effects on CD4+ regulatory T cells and GR-1+ macrophages at days 1, 7, 14 and 28 after injuries. Groups of male CD-1 mice were exposed to 0, 0.5, 1, 2, 4, 6 or 8 Gy radiation by timed exposure to 137CsCl without or with combined 25% TBSA burn injury. At days 1, 7, 14 or 28 after injuries, cells were prepared from lymph nodes and spleens from individual mice. Cell suspensions were stained with anti-CD4 and anti-FoxP3 antibodies to identify CD4 regulatory T cells (Tregs) and anti-F4/80 and anti-GR-1 to identify the GR-1+ macrophage subset in spleens. The (*) indicates significant differences between sham and burn injury and (#) indicates significant differences between 0 Gy radiation and radiation exposures at the indicated doses. P < 0.05 by two-way ANOVA and Bonferroni multiple comparisons for n = 5–6 individual mice per group.