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. Author manuscript; available in PMC: 2015 Jan 8.
Published in final edited form as: Am J Transplant. 2013 Sep 5;13(11):3021–3030. doi: 10.1111/ajt.12417

Figure 4. Anti-CD154 dAbs reduce numbers of functional antigen-specific cells and prevent differentiation into multiple cytokine producers.

Figure 4

(A) Representative flow plots of intracellular cytokine staining of splenic antigen-specific CD8+ T cells harvested at day 11 and stimulated for 4 hours in vitro with SIINFEKL peptide. (B) Percentages and absolute numbers of IFN-γ producing antigen-specific CD8+ T cells. Compared to DST alone, treatment with MR-1, 7E1-G2b or CD154 dAbs significantly reduced the frequency and absolute numbers of IFN-γ-producing antigen-specific CD8+ T cells in the spleen. (C) Frequencies of double cytokine producing antigen-specific CD8+ T cells within each treatment group. Treatment with MR-1, 7E1-G2b or CD154 dAbs resulted in the reduction of percentage of multicytokine producing antigen-specific CD8+ T cells. Data are representative of three independent experiments with a total of eleven to twelve mice per group. *** p < 0.001.