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. Author manuscript; available in PMC: 2015 Jan 8.
Published in final edited form as: J Immunol Methods. 2011 Sep 6;376(0):1–12. doi: 10.1016/j.jim.2011.08.023

Figure 5. Cytokine release following stimulation with anti-CD3ε mAb, SEB, or OVA-peptide.

Figure 5

Cytokine measurements in cell supernatants following stimulation were conducted to further validate the effectiveness of cell stimulation conditions used in our in vitro experiments. Splenocytes from WT or OT-II transgenic mice were incubated with anti-CD3ε mAb, SEB, or OVA-peptide at different doses (0, 0.01, 0.1, or 1 µg/ml) for 48 hours. Cytokines in the supernatant were measured using the Luminex cytokine multiplex assay. Stimulating cells with anti-CD3ε, SEB, or OVA-peptide caused a dose-dependent release of the cytokines IL-1α, IL-2, IL-6, IL-10, IFN-γ, and TNF-α. Data represent the mean ± SEM from 3 individual mice per group.