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. Author manuscript; available in PMC: 2015 Jan 8.
Published in final edited form as: J Immunol Methods. 2011 Sep 6;376(0):1–12. doi: 10.1016/j.jim.2011.08.023

Figure 6. Expression and phosphorylation of PKC-θ and ZAP-70 in Tregs vs. non-Tregs following in vivo challenge of mice with SEB.

Figure 6

WT mice were challenged by i.p injection with 25µg/mouse SEB, dissolved in 250µl of PBS, or sham-treated by injection of PBS alone. After 30 min, 1 hour, or 2 hours treated mice were sacrificed and lymph nodes (LN) and spleens (SPL) were harvested to generate cell suspensions. The expression and phosphorylation of PKC-θ and ZAP-70 was assessed in Tregs and non-Tregs by flow cytometry. Data shown represent one of two independent experiments. N=3 mice. Mean ± SEM given, #p<0.05 vs. sham (SPL), *p<0.05 vs. sham (LN).