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. 2014 Oct 14;15(1):897. doi: 10.1186/1471-2164-15-897

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© Kwon et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Capacitation status and tyrosine phosphorylation of spermatozoa following incubation in capacitation media. (A) Changes in capacitation pattern. Data represent mean ± SEM, n =3, *P <0.05. (B) Ratios of tyrosine phosphorylated proteins (optical density [OD] × mm)/α-tubulin (OD × mm). Data represent mean ± SEM, n =3, *P <0.05. The red line indicates the landmark of before-capacitation (BC). (C) Tyrosine-phosphorylated proteins were probed with anti-Phosphotyrosine (4G10) antibody; lane 1: before capacitation; lane 2: after capacitation.